工作学习经历

2017年12月-至今，湘潭大学化学学院，教授，博士生导师

2013-2014年，美国伊利诺伊大学香槟分校，访问学者

2008年-至今，湘潭大学化学学院，教师

2005-2008年，湖南大学化学化工学院，博士

2002-2005年，湖南大学化学化工学院，硕士

1998-2002年，河南师范大学化学化工学院，本科

主讲课程

分析化学、光谱分析、色谱分析

研究方向

有机小分子荧光探针的设计合成，纳米荧光探针的构建，荧光探针在生化分析中的应用

主要学术成果

承担项目（主持）

[1] 国家自然科学基金面上项目，糖类受体靶向性近红外荧光探针的构建及在炎症性肠病成像中的应用研究，2022-2025；

[2] 国家自然科学基金面上项目，基于缺氧诱导释放的近红外诊疗探针的构建及其初步生物医学应用研究，2018-2021；

[3] 国家自然科学基金青年项目，基于能量转移机理的蛋白激酶比率荧光分子探针的设计合成及生物医学应用，2011-2013；

[4] 湖南省自然科学基金项目面上项目，双模态成像探针的构建及在炎症性肠病早期诊断中的应用研究，2021-2023；

[5] 湖南省自然科学基金项目青年人才培养基金，定位线粒体的比率型三磷酸腺苷荧光探针的研究，2015-2017；

[6] 湖南省自然科学基金项目面上项目，能量转移型蛋白激酶荧光探针的研究，2011-2013；

[7] 中国博士后科学基金特别资助项目，定位线粒体的比率型荧光探针的研究，2015-2016；

[8] 中国博士后科学基金面上项目，能量转移型荧光探针的研究，2014-2016；

[9] 湖南省教育厅重点项目，缺氧诊疗荧光探针的研究，2019-2021；

[10] 湖南省教育厅优秀青年项目，用于三磷酸腺苷定位和检测的双功能比率荧光探针的构建及细胞成像研究，2016-2018；

[11] 湖南省教育厅一般项目，能量转移型蛋白激酶荧光探针的研究，2011-2013

部分发表论文（第一作者或通讯作者）

[58] Accurate fluorescence diagnosis of cancer based on sequential detection of hydrogen sulfide and pH, Analytical Chemistry, 2021, 93, 11826-11835. (IF: 6.986)

[57] Monitoring the fluctuation of hydrogen peroxide in diabetes and its complications with a novel near-infrared fluorescent probe, Analytical Chemistry , 2021, 93, 3301-3307. (IF: 6.986)

[56] Construction of NIR and ratiometric fluorescent probe for monitoring carbon monoxide under oxidative stress in zebrafish, Analytical Chemistry , 2021, 93, 2510-2518. (IF: 6.986)

[55] NAD(P)H-triggered probe for dual-modal imaging during energy metabolism and novel strategy of enhanced photothermal therapy in tumor, Biomaterials , 2021, 271, 120736. (IF: 12.479)

[54] A near-infrared fluorescence probe for accurately diagnosing cancer by sequential detection of cysteine and H+, Chemical Communications , 2021, 57, 4811-4814. (IF: 6.222)

[53] Real-time imaging of alkaline phosphatase activity of diabetes in mice via a near-infrared fluorescent probe, Chemical Communications, 2021, 57, 480-483. (IF: 6.222)

[52] A novel HPQ-based fluorescent probe for the visualization of carbon monoxide in zebrafish, Sensors and Actuators B: Chemical, 2021, 340, 129920. (IF: 7.460)

[51] A near-infrared fluorescent probe with large Stokes shift for imaging Cys in tumor mice, Analytica Chimica Acta, 2021, 1171, 338655. (IF: 6.558)

[50] Novel near-infrared fluorescence probe with large Stokes shift for monitoring CCl4-induced toxic hepatitis, Talanta , 2021, 223, 121720. (IF: 6.057)

[49] ATP-responsive near-infrared fluorescence MOF nanoprobe for the controlled release of anticancer drug. Microchimica Acta, 2021, 188, 287. (IF: 5.833)

[48] The construction of a near-infrared fluorescent probe with dual advantages for imaging carbon monoxide in cells and in vivo. Analyst , 2021, 146, 118-123. (IF: 4.616)

[47] Novel strategy for validating the existence and mechanism of the “gut-liver axis” in vivo by a hypoxia-sensitive NIR fluorescent probe, Analytical Chemistry , 2020, 92, 4244-4250. (IF: 6.986)

[46] Near-infrared fluorescence MOF nanoprobe for adenosine triphosphate-guided imaging in colitis, ACS Applied Materials & Interfaces , 2020, 12, 47840-47847. (IF: 9.229)

[45] Near-infrared fluorescence probe with a large stokes shift for visualizing hydrogen peroxide in ulcerative colitis mice, Sensors and Actuators B: Chemical , 2020, 320, 128296. (IF: 7.460)

[44] A novel hepatocyte-targeting ratiometric fluorescent probe for imaging hydrogen peroxide in zebrafish, Sensors and Actuators B: Chemical , 2020, 313, 128054. (IF: 7.460)

[43] A near-infrared fluorescence probe with a large Stokes shift for detecting carbon monoxide in living cells and mice, Dyes and Pigments , 2020, 180, 108517. (IF: 4.889)

[42] A facile pH near-infrared fluorescence probe for the diagnosis of cancer in vivo, Dyes and Pigments , 2020, 179, 108402. (IF: 4.889)

[41] In-situ imaging of azoreductase activity in the acute and chronic ulcerative colitis mice by a near-infrared fluorescent probe. Analytical Chemistry , 2019, 91, 10901-10907. (IF: 6.986)

[40] Fluorescence-guided cancer diagnosis and surgery by a zero cross-talk ratiometric near-infrared γ-glutamyltranspeptidase fluorescent probe. Analytical Chemistry , 2019, 91, 1056-1063. (IF: 6.986)

[39] A hepatocyte-targeting near-infrared ratiometric fluorescent probe for monitoring peroxynitrite during drug-induced hepatotoxicity and its remediation. Chemical Communications , 2019, 55, 14307-14310. (IF: 6.222)

[38] A rhodamine-deoxylactam based fluorescent probe for fast and selective detection of nitric oxide in living cells. Talanta , 2019, 197, 436-443. (IF: 6.057)

[37] A ratiometric fluorescent probe for the detection of peroxynitrite with simple synthesis and large emission shift and its application in cells image. Dyes and Pigments , 2019, 161, 288-295. (IF: 4.889)

[36] A dual-response fluorescent probe for the detection of viscosity and H2S and its application in studying their cross-talk influence in mitochondria. Analytical Chemistry , 2018, 90, 9418-9425. (IF: 6.986)

[35] A ratiometric fluorescence probe for peroxynitrite prepared by denovo synthesis and its application in assessing mitochondrial oxidative stress status in cells and in vivo. Chemical Communications , 2018, 54, 11590-11593. (IF: 6.222)

[34] Efficient two-photon fluorescent probe for imaging of nitric oxide during endoplasmic reticulum stress. ACS Sensors , 2018, 3, 2311-2319. (IF: 7.711)

[33] Detecting and imaging of γ-glutamytranspeptidase activity in serum, live cells and pathological tissues with a high signal stability probe by releasing a precipitating fluorochrome. ACS Sensors , 2018, 3, 1354-1361. (IF: 7.711)

[32] Two-photon fluorescence probe for precisely detecting endogenous H2S in lysosome by employing a dual lock system. Sensors and Actuators B: Chemical , 2018, 260, 264-273. (IF: 7.460)

[31] An infinite coordination polymer nanoparticles-based near-infrared fluorescent probe with high photostability for endogenous alkalinephosphatase in vivo. Sensors and Actuators B: Chemical , 2018, 255, 3355-3363. (IF: 7.460)

[30] Mitochondria-targeted near-infrared fluorescent probe for the detection of carbon monoxide in vivo. Talanta , 2018, 188, 691-700. (IF: 6.057)

[29] A facile and sensitive near-infrared fluorescence probe for the detection of endogenous alkaline phosphatase activity in vivo. Analytical Chemistry , 2017, 89, 6854-6860. (IF: 6.986)

[28] Real-time monitoring ATP in mitochondrion of living cells: a specific fluorescent probe for ATP by dual recognition sites. Analytical Chemistry , 2017, 89, 1749-1756. (IF: 6.986)

[27] A rhodamine-based fluorescent probe with high water solubility and its application in the detection of glutathione with unique specificity. Sensors and Actuators B: Chemical , 2017, 240, 1165-1173. (IF: 7.460)

[26] A near-infrared fluorescent probe based on BODIPY derivative with high quantum yield for selective detection of exogenous and endogenous cysteine in biological samples. Analytica Chimica Acta , 2017, 994, 73-81. (IF: 6.558)

[25] Borondipyrrolemethene-based fluorescent probe for distinguishing cysteine from biological thiols and its application in cell image. Dyes and Pigments , 2017, 139, 381-387. (IF: 4.889)

[24] A near-infrared chemosensor for determination of trivalent aluminum ions in living cells and tissues. Dyes and Pigments , 2017, 136, 817-824. (IF: 4.889)

[23] A borondipyrrolemethene-based turn-on fluorescent probe for silver ion with high sensitivity and selectivity and its application in water samples and living cells. Tetrahedron Letters , 2017, 58, 3536-3540. (IF: 2.415)

[22] Near-infrared fluorescent probe with high quantum yield and its application in the selective detection of glutathione in living cells and tissues. Analytical Chemistry , 2016, 88, 9746-9752. (IF: 6.986)

[21] Rhodamine-based chemosensor for fluorescence determination of trivalent chromium ion in living cells. Sensors and Actuators B: Chemical , 2016, 223, 705-712. (IF: 7.460)

[20] Development of a simple pyrene-based ratiometric fluorescent chemosensor for copper ion in living cells. Sensors and Actuators B: Chemical , 2016, 222, 1226-1232. (IF: 7.460)

[19] Rhodamine-based chemodosimeter for fluorescent determination of Hg2+ in 100% aqueous solution and in living cells. Analytica Chimica Acta , 2016, 934, 218-225. (IF: 6.558)

[18] A highly sensitive and selective fluorescent probe for trivalent aluminum ion based on rhodamine derivative in living cells. Analytica Chimica Acta , 2015, 888, 155-161. (IF: 6.558)

[17] A ratiometric fluorescent probe with unexpected high selectivity for ATP and its application in cell imaging. Chemical Communications , 2014, 50, 15411-15414. (IF: 6.222)

[16] A ratiometric fluorescent chemosensor for Cr3+ based onmonomer-excimer conversion of a pyrene compound. Sensors and Actuators B: Chemical , 2014, 203, 712-718. (IF: 7.460)

[15] Ratiometric near-infrared chemosensor for trivalent chromium ion based on tricarboyanine in living cells. Analytica Chimica Acta , 2014, 824, 71-77. (IF: 6.558)

[14] A new rhodamine-based fluorescent chemosensor for Fe3+ and its application in living cell imaging. Dyes and Pigments , 2014, 104, 110-115. (IF: 4.889)

[13] Efficient FRET-based colorimetric and ratiometric fluorescent chemosensor for Al3+ in living cells. Sensors and Actuators B: Chemical , 2013, 186, 360-366. (IF: 7.460)

[12] A colormetric and fluorescent chemosensor for adenosine-5’-triphosphate based on rhodamine derivative. Analytica Chimica Acta , 2013, 795, 69-74. (IF: 6.558)

[11] Colorimetric and fluorescent chemosensor for citrate based on a rhodamine and Pb2+ complex in aqueous solution. Analytica Chimica Acta , 2013, 774, 79-84. (IF: 6.558)

[10] Ratiometric and colorimetric fluorescent chemosensor for Ag+ based on tricarbocyanine. Dyes and Pigments , 2013, 99, 903-907. (IF: 4.889)

[9] A fluorescent chemosensor for Hg2+ based on a rhodamine derivative in an aqueous solution. Analytical Sciences , 2013, 29, 899-903. (IF: 2.081)

[8] A fluorescent chemosensor for Hg2+ based on naphthalimide derivative by fluorescence enhancement in aqueous solution. Analytica Chimica Acta , 2012, 717, 122-126. (IF: 6.558)

[7] A fluorescent pH chemosensor based on functionalized naphthalimide in aqueous solution. Analytical Sciences , 2012, 28, 743-747. (IF: 2.081)

[6] A fluorescent chemosensor for cysteine based on naphthalimide derivative in aqueous solution. Sensors and Actuators B: Chemical , 2011, 155, 253-257. (IF: 7.460)

[5] A fluorescent chemosensor for silver ions based on porphyrin compound with high selectivity. Spectrochimica Acta Part A , 2010, 76, 197-201. (IF: 4.098)

[4] Naphthalimide-porphyrin hybrid based ratiometric bio-imaging probe for Hg2+: well-resolved emission spectra and unique specificity. Analytical Chemistry , 2009, 81, 9993-10001. (IF: 6.986)

[3] A porphyrin derivative containing 2-(oxymethyl)pyridine units showing unexpected ratiometric fluorescent recognition of Zn2+ with high selectivity. Analytica Chimica Acta , 2008, 616, 214-221. (IF: 6.558)

[2] A fluorescent chemosensor for cobalt ions based on a multi-substituted phenol-ruthenium (II) tris(bipyridine) complex. Analytica Chimica Acta , 2006, 580, 143-148. (IF: 6.558)

[1] A wide pH range optical sensing system based on a sol-gel encapsulated amino-functionalisedcorrole. Analyst , 2006, 131, 388-393. (IF: 4.616)

授权专利

[11] 一种过氧亚硝基比率型荧光探针的制备和应用，专利号ZL201910474219.8

[10] 基于脱氧罗丹明的一氧化氮荧光探针的制备和应用，专利号ZL201811120191.X

[9] 基于半花菁染料的过氧化亚硝基荧光探针的制备和应用，专利号ZL201810233068.2

[8] 一种近红外碱性磷酸酶荧光探针的制备方法和应用，专利号ZL201611230342.8

[7] 一种近红外GSH荧光探针的制备方法和应用，专利号ZL201610321434.0

[6] 基于罗丹明的GSH水溶性荧光探针的制备方法和应用，专利号ZL201610821181.3

[5] 一种基于氟硼吡咯的Cys荧光探针的制备方法和应用，专利号ZL201610902695.1

[4] 一种定位线粒体的ATP荧光探针的制备及应用，专利号ZL201610929418.X

[3] 基于罗丹明水溶性汞离子荧光探针的制备方法和应用，专利号ZL201610250556.5

[2] 一种基于芘的铜离子比率型荧光探针及其制备方法和应用，专利号ZL201410846656.5

[1] 一种铬离子比率型荧光探针及其制备方法和应用，专利号ZL201310322506.X

研究生培养

[1] 在读：江文丽（博士），王文新（博士），王之卿（博士），侯美家，闫灵，廖沁婷，付贵勤，顾青松，陈骏涛，钞静静，李婷，陈思思，张会，胡玲，秦崇康

[2] 已毕业：刘娟，夏雨桑，佘遵攀，陈茜茜，赵仪婷，田杨（校长奖），张烁，周东叶，欧阳娟（国家奖学金，校长奖特等奖，湖南省优秀硕士学位论文），李宋娇（国家奖学金），谢俊英（国家奖学金，校长奖），谭凯月，付雅俊，吴银双（国家奖学金），李丹，李芝，唐佳良（国家奖学金），卢曦，邹春香（国家奖学金），孔雪飞（校长奖），周雨（校长奖），徐芬（校长奖）

个人简介

李春艳，教授，博士生导师，2008年博士毕业于湖南大学化学化工学院。2019年入选中国化学会“中国青年化学家元素周期表”，并代言“汞”元素。2013年入选首届湘潭大学青年拔尖人才计划，湘潭大学韶峰学者学术骨干。主要从事有机小分子探针设计合成、纳米荧光探针的构建和荧光探针在生化分析中的应用研究。主持国家自然科学基金面上项目/青年项目、中国博士后基金面上项目/特别资助项目、湖南省自然科学基金、湖南省教育厅重点项目/优秀青年项目等课题。近年来以第一作者或通讯作者在Analytical Chemistry、Chemical Communication、Biomaterials、ACS Applied Materials & Interfaces、ACS Sensors等国际刊物发表论文近60篇，其中IF>6.0的论文40多篇，论文他引2400多次。获授权的发明专利11项。已培养研究生40人，毕业25人，在读15人（其中博士3人），指导研究生多次获得研究生国家奖学金、湖南省优秀硕士学位论文、湘潭大学“研究生校长奖”等荣誉。